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Generation of human antibody fragments against <Emphasis Type="Italic">Streptococcus mutans</Emphasis>using a phage display chain shuffling approach |
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| Authors: | Michael?B?Kupper Michael?Huhn Holger?Spiegel Julian?KC?Ma Stefan?Barth Rainer?Fischer |
| Institution: | (1) Fraunhofer Institute for Molecular Biology and Applied Ecology, 52074 Aachen, Germany;(2) Technical University Aachen, Department of Molecular Biotechnology, 52074 Aachen, Germany;(3) Department of Cellular and Molecular Medicine, Molecular Immunology Unit, St. George's Hospital Medical School, London, SW17 ORE, UK |
| Abstract: | BackgroundCommon oral diseases and dental caries can be prevented effectively by passive immunization. In humans, passive immunotherapy may require the use of humanized or human antibodies to prevent adverse immune responses against murine epitopes. Therefore we generated human single chain and diabody antibody derivatives based on the binding characteristics of the murine monoclonal antibody Guy's 13. The murine form of this antibody has been used successfully to prevent Streptococcus mutans colonization and the development of dental caries in non-human primates, and to prevent bacterial colonization in human clinical trials. |
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