| 神经生长因子的发光免疫分析法 |
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| 引用本文： | 刘农乐,蒋滋慧,柳川,赵强,陈艾,李美佳,王会信,周廷冲. 神经生长因子的发光免疫分析法[J]. 中国生物化学与分子生物学报, 1994, 10(5): 616-620 |
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| 作者姓名： | 刘农乐 蒋滋慧 柳川 赵强 陈艾 李美佳 王会信 周廷冲 |
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| 作者单位： | 军事医学科学院基础医学研究所,中国医学科学院放射医学研究所 |
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| 摘 要： | 介绍了一种灵敏的神经生长因子化学发光免疫测定方法。小鼠神经生长因子（ｍＮＧＦ）的抗体ＩｇＧ用亲和层析纯化并用吖啶酯标记。该法可用于大鼠组织中ＮＧＦ含量的测定。方法的灵敏度为１０ｐｇ／ｍＬＮＧＦ;批内批间变异系数分别为８．７％及１３．２％;回收率平均为１０３％。ｍＮＧＦ抗体对人的ＮＧＦ也有极强的交叉反应,故本方法也可能用于病人血清或脑脊液中ＮＧＦ含量的测定。
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| 关 键 词： | 神经生长因子 吖啶酯 化学发光免疫分析法, |
| 收稿时间： | 1994-10-20 |
Chemiluminescence Immunoassay for Nerve Growth Factor |
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| Liu,Nong-le,Jiang,Zi-hui,Liu,Chuan,Zhao,Qiang,Chen,Ai,Li,Mei-jia,Wang,Hui-xin,Zhou,Ting-chong. Chemiluminescence Immunoassay for Nerve Growth Factor[J]. Chinese Journal of Biochemistry and Molecular Biology, 1994, 10(5): 616-620 |
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| Authors: | Liu Nong-le Jiang Zi-hui Liu Chuan Zhao Qiang Chen Ai Li Mei-jia Wang Hui-xin Zhou Ting-chong |
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| Affiliation: | (Institure of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850)(Institute of Radiation Medicine,Chinese Academy of Medical Sciences,Ti |
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| Abstract: | A sensitive chemiluminescence assay for nerve growth factor was described. Antibody IgG for mouse nerve growth factor (mNGF) was purified by affinity chromatography and labeled with acridinium ester. NGF contents of rat tissues were determined by this method. The sensitivity of the assay was 10pg/mL NGF. The intra and inter coefficients of variation were 8.7% and 13. 2% respectively. Recovery tests were determined to be 103%. High degree cross reaction between mNGF antibody and human NGF was observed. It may be possible to determine the NGF levels in serum or cerebrospinal fluid of patients by this method. |
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| Keywords: | Nerve growth factor Acridinium ester Chemiluminescence assay |
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