Spatiotemporal Expression of PSD-95 and nNOS After Rat Sciatic Nerve Injury |
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Authors: | Shangfeng Gao Min Fei Chun Cheng Xiaowei Yu Mengling Chen Shuxian Shi Jing Qin Zhiqin Guo Aiguo Shen |
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Institution: | (1) The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, China;(2) Department of Microbiology and Immunology, Medical School of Nantong University (Former Nantong Medical College), Nantong, 226001, China;(3) Institute of Medical Biotechnology, Soochow University, Suzhou, 215007, China;(4) Soochow University & Saier Immuno-Biotech Co. Ltd., Suzhou, 215123, China |
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Abstract: | Neuronal nitric oxide synthase (nNOS) has been implicated to influence peripheral nerve lesion and regeneration. Post-synaptic
density-95 (PSD-95) is one of nNOS-anchoring proteins and plays an important role in specifying the sites of reaction of NO
in nervous system. Here we established a rat sciatic nerve crush (SNC) model to examine the spatiotemporal expression of PSD-95
and nNOS. At gene levels, PSD-95 mRNA diminished shortly after crush, and significantly elevated from 2 days to 2 weeks, whereas
nNOS decreased progressively post-operation, reached the valley at 1 day, and markedly up-regulated from 1 to 2 weeks after
SNC. The expression of both molecules returned to the control level at 4 weeks post-injury. At protein levels, PSD-95 and
nNOS underwent the similar changes as their gene expression except for a time lag during up-regulating. At their peak expression,
PSD-95 co-labeled with nNOS in Schwann cells (SCs) of sciatic nerve within 0.5 mm from the lesion site, but had few colocalization
in axons. In addition, the interaction between PSD-95 and nNOS enhanced significantly at 2 weeks after SNC. These results suggest
a correlation of PSD-95 up-regulation with nNOS in reactive SCs of crushed sciatic nerve, which may lead to understanding
the function of PSD-95 during peripheral nerve regeneration.
Shangfeng Gao and Min Fei contributed equally to this work. |
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Keywords: | Post-synaptic density-95 Neuronal nitric oxide synthase Sciatic nerve crush Coimmunoprecipitation Immunofluorescence Rat |
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