Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP |
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| Authors: | Biteen Julie S Thompson Michael A Tselentis Nicole K Bowman Grant R Shapiro Lucy Moerner W E |
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| Institution: | Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, USA. |
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| Abstract: | The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging. |
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