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Physiological functions of hemocytes newly emerged from the cultured hematopoietic organs in the silkworm, Bombyx mori |
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| Authors: | Cheng-Long Wang Zhi-Xiang Wang Michael M Kariuki Qing-Zhi Ling Kenji Kiguchi Er-Jun Ling |
| Institution: | Research Centre for Insect Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China;, Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA;, Department of Applied Biology, Zhejiang Pharmaceutical College, Ningbo, Zhejiang Province, China;, and Laboratory of Silkworm Physiology, Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan |
| Abstract: | Abstract Cellular immunity is a very important part of insect innate immunity. It is not clear if hemocytes entering the hemolymph require a maturation process to become competent. The establishment of a tissue culture system for the insect hematopoietic organs would enable physiological function assays with hemocytes newly emerged from hematopoietic organs. To this end, we established a hematopoietic organ culture system for the purebred silkworm pnd pS and then studied the physiological functions of the newly emerged hemocytes. We found that Grace's medium supplemented with 10% heated silkworm larval plasma was better for culturing the hematopoietic organs of pnd pS . Newly emerged hemocytes phagocytosed propidium iodide-labeled bacteria and encapsulated the Iml-2 coated nickel beads as well as pupal tissue debris. This culture system is therefore capable of generating physiologically functional hemocytes. These hemocytes can be used to study the mechanisms of the hemocyte immune response among others. |
| Keywords: | Bombyx mori encapsulation hemocyte hematopoietic organ phagocytosis tissue culture |