Production of additional allotetraploid somatic hybrids combining mandarings and sweet orange with pre-selected pummelos as potential candidates to replace sour orange rootstock |
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Authors: | G Ananthakrishnan M ?alovi? P Serrano J W Grosser |
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Institution: | (1) IFAS, Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, 33850 Lake Alfred, FL |
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Abstract: | Summary Sour orange (Citrus aurantium L.) rootstock has historically been a widely utilized eitrus rootstock throughout the world due to its wide soil adaptability
and superior horticultural performance. However, quick-decline isolates of citrus tristeza virus (CTV) have demolished entire
industries of sour orange rootstock in some countries, including Brazil and Venezuela. CTV is presently destroying millions
of trees of sour orange rootstock in Florida and threatens the citrus industries of Texas and Mexico, where sour orange is
the predominant rootstock. Efforts to replace sour orange rootstock are combining traditional breeding and biotechnology approaches,
including somatic hybridization and transformation. Molecular techniques have confirmed that sour orange is probably a hybrid
of mandarin and pummelo. A major focus of our program continues to be the somatic hybridization of superior mandarins with
pre-selected pummelo parents. Here, we report the regeneration of allotetraploid somatic hybrid plants from seven new mandarin+pummelo
combinations and one new sweet orange+pummelo combination. All new somatic hybrids were confirmed by leaf morphology, ploidy
analysis via flow cytometry, and random amplified polymorphic DNA analysis to show nuclear contributions from both parents
in corresponding hybrids. These new somatic hybrids are being propagated by tissue culture and/or rooted cuttings for further
evaluation of disease resistance and horticultural performance in field trials. |
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Keywords: | citrus tissue culture citrus tristeza virus (CTV) disease resistance protoplast fusion somatic embryogenesis |
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