| 用免疫共沉淀进一步确定SMAD4中间连接区在形成同源和异源复合物时的作用 |
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| 引用本文： | 刘一平,邓继先,张英丽,吕娅歆,程萱,黄翠芬. 用免疫共沉淀进一步确定SMAD4中间连接区在形成同源和异源复合物时的作用[J]. 中国生物化学与分子生物学报, 2000, 16(4): 537-541 |
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| 作者姓名： | 刘一平 邓继先 张英丽 吕娅歆 程萱 黄翠芬 |
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| 作者单位： | 1. 军事医学科学院生物工程研究所北京 100071
2. 白求恩医科大学第三临床医院 |
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| 摘 要： | 用酵母双杂交系统发现 SMAD4的中间连接区能与 SMAD3相互作用 ,而 SMAD4的 N区和 C区不能与 SMAD3相互作用 ,此结果与前人报道的结果有出入 .用细胞免疫共沉淀的方法进一步证实此现象 .结果与酵母双杂交的结果完全吻合 .说明 SMAD4与 SMAD3相互作用形成异源复合物时确实是通过 SMAD4的中间连接区实现的
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| 关 键 词： | SMAD4 SMAD3 免疫共沉淀 |
| 收稿时间： | 2000-08-20 |
| 修稿时间： | 1999-09-03 |
Further Identification of the Function of SMAD4 Linker Region in Homomeric and Heteromeric Complexes with Co-immunoprecipitation |
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| LIU Yi|ping,DENG Ji|xian,ZHANG Ying|li,LU Ya|xin,CHENG Xuan,HUANG Cui|fen. Further Identification of the Function of SMAD4 Linker Region in Homomeric and Heteromeric Complexes with Co-immunoprecipitation[J]. Chinese Journal of Biochemistry and Molecular Biology, 2000, 16(4): 537-541 |
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| Authors: | LIU Yi|ping DENG Ji|xian ZHANG Ying|li LU Ya|xin CHENG Xuan HUANG Cui|fen |
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| Affiliation: | (Bejing Institute of Biotechnology,Beijing 100071,China |
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| Abstract: | It is suggested that the linker region of SMAD4 has no function in the heteromeric and homomeric interaction of SMAD4.But in the previous research with yeast two|hybrid assay,it was observed that the linker region of SMAD4 played a key role in the heteromeric interactions of SMAD4 and SMAD3,and in the homomeric interaction of SMAD4.It verified the function of SMAD4 linker region in heteromeric and homomeric interaction with co|immunoprecipitation.The result showed that the SMAD4 linker region did play essential role in the interactions. |
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| Keywords: | SMAD4 SMAD3 co|immunoprecipitation |
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