Lipid peroxidation during low-temperature adaptation of cold-sensitive tobacco leaves and roots

We studied low-temperature adaptation of cold-sensitive tobacco plants in relation to peroxidation of lipids (POL) in their leaves and roots. Experiments were performed with tobacco plants (Nicotiana tabacum L., cv. Samsun). Cold hardening (6 days at 8°C) exerted principally different action on tobacco leaves and roots. In the leaves, the contents of dienoic conjugates and MDA was reduced, and tissue cold tolerance, even to below zero temperatures, was improved. In contrast, in the roots, POL was activated and root cold tolerance decreased. It is suggested that an incapability of the tobacco root system to adapt to low temperature was a limiting factor determining the low potential of this and other cold-sensitive plants to hypothermia.