Comparison of sequence homology of poly(A) and non-poly(A) containing 34S RNA of AKR murine leukemia virus.

AKR MuLV 70S RNA was separated on Poly(U)-Sepharose into poly(A) and non-poly(A) containing 34S subunits. The ratio of the two fractions was 2:1, respectively. Both fractions were hybridized to AKR MuLV [³H]cDNA, and the hybrids were assayed by nuclease S₁ and cesium sulfate centrifugation. The poly(A) and non-poly(A) subunits hybridized to [³H]cDNA to the same extent (80%), with identical $\text{CO}{}_{\mathrm{<mtext>1</mtext><mtext>2</mtext>}}$ values; and the hybrids of both fractions had identical T_m values (81°C in 0.15 M NaCl). These results demonstrate that the poly(A) and non-poly(A) containing subunits of the AKR genome have identical or very similar base sequences in the heteropolymeric regions.