城市生活污水用于培养雨生红球藻的研究

虾青素是自然界广泛存在的一种橘红色类胡萝卜素，广泛应用于食品、药品和化妆品行业。在虾青素的制备中，雨生红球藻是生产虾青素的最有效来源，目前提高虾青素产量的方式主要为提高生物量和产物合成率。目前已有大量研究针对生物量的优化，但依然存在改善空间。为此，尝试用城市生活污水作为培养基对雨生红球藻进行培养。结果表明，生活污水能促进雨生红球藻的生长，其产量是现有BG11培养基的2倍；虾青素的合成时期显著提前（P<0.05），且体内重金属含量未明显富集，处在安全浓度范围。此外，养藻后的城市生活污水中氮、磷含量显著降低（P<0.05），高氮、磷富余的情形得到有效改善。证实利用污水培养雨生红球藻的双重效应，一方面有利于积累藻类生物量，另一方面有助于净化水质，在经济效益和生态效益上具有极好的发展潜力。

Cultivation of Haematococcus pluvialis on Urban Sewage

Astaxanthin, a red-orange carotenoid pigment, is a powerful biological antioxidant that occurs naturally in a wide variety of living organisms. As a valuable natural product, astaxanthin was widely used in food and cosmetics industry, and considerable potential applications in human health and clinical medicine. Among the acquire process of astaxanthin, Haematococcus pluvialis is the most effective biological source. There are two ways to increase the production of astaxanthin:increasing the biomass and improving the synthesis efficiency. Previously research works have obtained many positive results, there is still a long-way to achieve the highest cost performance ratio. Thus, the municipal wastewater as medium to culture the H. pluvialis is used. The results shows that swage can improve the growth of H. pluvialis greatly, and the biomass is over 2-fold than the control medium (BG11 medium). After detect the level of heavy metal content in the H. pluvialis cell, there was no significant heavy metal enrichment in the algal body. The hazardous metal concentrations in the safe range. In addition, the new develop method can accelerate the products synthetise, and made the synthetic period of astaxanthin advanced significantly (P<0.05). Besides, it is worth noting that the content of nitrogen and phosphorus in the sewage reduced significantly with the growth of algae (P<0.05), eutrophic wastewater has been efficiently improved. Tanken together, our experiment demonstrate the feasibility to culture H. pluvialis by sewage. It can bring double effects:accumulation algal biomass, and helpful to purify water quality. The excellent economic and ecological benefits in algae culturing industry can be gained.