诱导多能干细胞技术的优化及其应用前景

诱导多能干细胞(induced pluripotent stem cells, iPSCs)是类似胚胎干细胞的一种细胞类型,可以通过对已分化的体细胞进行诱导重编程获得,具有自我更新能力和多潜能性,在体外疾病模型的建立、移植替代治疗、发育学等方面有广阔的应用前景,但致瘤性、转化率低、疾病模型拟合度差等缺点限制着iPS技术在临床和科研上的推广。对近几年诱导多能干细胞技术优化方面取得的新进展进行综述,重点阐述降低致瘤性和提高转化率的几种方法及iPS在临床和科研上的应用前景。

Optimization of Induced Pluripotent Stem Cell Technology and Its Application Prospect

Induced pluripotent stem cells (iPSCs) are a type of cells similar to embryonic stem cells (ESCs) which have the characters of self-renewal and pluripotency.They can be obtained through the induced reprogramming of differentiated somatic cells.iPSC has broad application prospects in the establishment of disease models in vitro,regenerative medicine,embryology etc. The controversies and obstacles that challenge the field are its tumorigenicity,low transformation efficiency and the low degree of fitting of the disease models. The majorizations of method on reprogramming were summarized, and the ways to increase transformation efficiency and reduce tumorigenicity were emphasized.The application prospects of iPS in clinical therapy and scientific research in the future were explored