Surface sugar binding components of bovine spermatozoa as evidenced by fluorescent neoglycoproteins |
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| Authors: | F Sinowatz H -J Gabius W Amselgruber |
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| Institution: | (1) Institute for Veterinary Anatomy II, Ludwig-Maximilians-University Munich, Veterinärstrasse 13, D-8000 München 22, Federal Republic of Germany;(2) Max-Planck-Institute for Experimental Medicine, Göttingen, Federal Republic of Germany |
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| Abstract: | Summary In the present study, the topographical distribution of carbohydrate binding sites on the plasma membrane of bovine epididymal spermatozoa was investigated using 15 different fluorescent neoglycoproteins and asialoglycoproteins. With mannose-bovine serum albumin (BSA)-fluoresceinthiocarbamyl (FTC), mannose-6-phosphate-BSA-FTC, lactose-BSA-FTC, maltose-BSA-FTC, asialolactoferrin-FTC and asialotransferrin-FTC a marked fluorescence was observed in the postacrosomal area. These results further substantiate the concept that carbohydrate binding sites of the spermatozoan plasma membrane and corresponding carbohydrates of the zona pellucida play a significant role in gamete interactions.Dedicated to Professor Dr. T.H. Schiebler on the occasion of his 65th birthday. |
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