Profilin I colocalizes with speckles and Cajal bodies: a possible role in pre-mRNA splicing |
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Authors: | Skare Petra Kreivi Jan-Peter Bergström Asa Karlsson Roger |
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Institution: | Department of Cell Biology, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden. |
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Abstract: | Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nuclear ribonucleoprotein particle (snRNP)-core proteins and p80 coilin-containing Cajal bodies. Treatment with actinomycin D led to largely similar reorganizations of snRNPs and profilin, while profilin and Cajal bodies separated under these conditions. One of the profilin antibodies interfered with pre-mRNA splicing in vitro, further indicating a role for profilin during pre-mRNA processing. |
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Keywords: | Profilin Nuclear distribution Pre-mRNA splicing Sm proteins |
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