A ribosomal DNA probe to distinguish populations of Rhopalosiphum maidis (Homoptera: Aphididae) |
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| Authors: | R. LUPOLI,M. E. IRWIN&dagger ,C. R. VOSSBRINCK&dagger |
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| Affiliation: | INRA-ENSAM Zoologie, place Viala, 34060 Montpellier Cedex 1, France;†University of Illinois and Illinois Natural History Survey, 607 East Peabody Drive, Champaign, IL 61820, USA |
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| Abstract: | Total DNA from clones of Rhopalosiphum maidis was digested with restriction endonucleases and hybridised with a heterospecific ribosomal DNA probe. DNA banding patterns exhibited sufficient differences to distinguish five different clones, and provided greater resolving power than gel electrophoresis of allozymes. The mechanisms and applications of this marking technique are discussed. |
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| Keywords: | Rhopalosiphum maidis genetic marker ribosomal DNA probe aphid clone migration |
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