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Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant <Emphasis Type="Italic">Lactococcus lactis</Emphasis> for oral vaccination and its protective immune response in mice |
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| Authors: | Chun Xia Hu Zi Rong Xu Wei Fen Li Niu Dong Ping Lu Ling Lin Fu |
| Institution: | (1) Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310029, People’s Republic of China;(2) Department of Life Science, Yuanpei College, Shaoxing University, Shaoxing, Zhejiang, 312000, People’s Republic of China;(3) Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, 310029, People’s Republic of China |
| Abstract: | K88 (F4) fimbrial adhesin, FaeG, was expressed extracellularly in Lactococcus lactis using a nisin-controlled gene expression system. The antibody response and protective efficacy of the recombinant bacteria (L. lactis spNZ8048-faeG]) against live enterotoxigenic E. coli (ETEC) C83549 challenge were evaluated in ICR mice. Mice vaccinated with L. lactis spNZ8048-faeG] had a significantly increased antigen-specific IgG level in the serum and decreased mortality rate (P < 0.05) compared with the control. This indicates that oral immunization of L. lactis spNZ8048-faeG] can induce an immune-response protection upon challenge with live ETEC in ICR mice. An erratum to this article can be found at |
| Keywords: | Enterotoxigenic Escherichia coli FaeG Immune response Lactococcus lactis Oral vaccination |
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