肝硬化小鼠肠道菌群结构及血清炎性因子的变化简

目的：观察肝硬化小鼠肠道菌群结构及血清炎性因子水平的变化。方法：通过CCL灌胃构建小鼠的肝硬化模型；采用酶联免疫吸附法检测肝硬化进程中血清内毒素及炎性因子IL-1、IL-6、TNF-α水平的变化；采集肝硬化小鼠新鲜粪便，通过荧光定量PCR实验检测肠道目的菌群的改变。结果：肝硬化小鼠肠道中拟杆菌属和梭菌属细菌显著减少，韦荣球菌属、肠杆菌属和肠球菌属细菌显著增加；随着肝硬化进程的加重，小鼠血液中内毒素及炎性因子水平显著提高。结论：肝硬化导致小鼠肠道菌群失调，促进了血液中内毒素及炎性因子水平的提高，形成恶性循环。

Changes of Intestinal Microbial Community and Serum Inflammato ry Factors in Cirrhosis Mice

Objective： To study the intestinal microbial community and serum inflammatory factors in cirrhosis mice. Methods： The cirrhotic mice models were constructed, then they were subjected to ELISA and real time PCR to detect endotoxin and inflammatory factors including IL-1, IL-6 and TNF-ot detection in serum and intesti nal microhiota respectively. Results： Cirrhotic mice had an increased abundance in VeilloneUa, Enterobacter, Entero coccus and a decreased abundance in Bacaeroides and Clostridium. In addition, serum inflammatory and endotoxin level were significantly increased in cirrhosis progress. Conclusion： The imbalanced intestinal microbial community in cirrhotic mice improved the increase in serum inflammatory and endotoxin level, which formed a vicious cycle.