| 白细胞介素－2的PEG化 |
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| 引用本文： | 常远,唐微,郑仲承,刘新垣.白细胞介素－2的PEG化[J].中国生物化学与分子生物学报,1996,12(4):445-449. |
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| 作者姓名： | 常远 唐微 郑仲承 刘新垣 |
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| 作者单位： | 中国科学院上海生物化学研究所 |
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| 摘 要： | 用自制的氨基ＰＥＧ化试剂ｒＩＬ－２进行化学修饰，研究了试剂浓度，溶液ｐＨ，反应时间等与ＰＥｃａ－ｒＩＬ－２产率及ＩＬ－２活性保持之间的关系，建立了一套获得稳定修饰度的ＰＥＧ－ｒＩＬ－２的方法。研究发现，反应时间跟修饰度关系不大；溶液ｐＨ对修饰度有一定的影响，中性ｐＨ以上反应都可进行；而试剂浓度直接决定修饰度的高低，过量越多，修饰度越高，而生物活性保留也越低；但低度修饰，对活性几乎没有影响，可保留活性在９５％左右。
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| 关 键 词： | 白细胞介素－2 PEG化 化学修饰 |
| 收稿时间： | 1996-08-20 |
Modification of Interlcukin-2 with Polyethylene Glycol |
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| Chang Yuan,Tang Wei,Zheng Zhong-Chen,Liu Xin-Yuan.Modification of Interlcukin-2 with Polyethylene Glycol[J].Chinese Journal of Biochemistry and Molecular Biology,1996,12(4):445-449. |
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| Authors: | Chang Yuan Tang Wei Zheng Zhong-Chen Liu Xin-Yuan |
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| Institution: | (Shanghai Institute of Biochemistry,Academia Sinca,Shanghai 200031 |
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| Abstract: | Interleukin-2 modified with activated PEG had been prepared.The relationship between reagent concentration,pH,reaction time etc.and protein modification degree or bioactivity reserve ratio of protein were studied.The results showed that the reaction time nearly do not effect the degree of modification,but pH may cause some effect on it.The higher reagent concentration can lead to higher modification degree but lower reserved bioactivity,while the PEG-IL-2 with 1,2 or 3PEG molecules retain>95% activity of IL-2. |
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| Keywords: | Interleukin-2 PEGylation Chemical modification |
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