Phylogenetic and metabolic bacterial diversity of <Emphasis Type="Italic">Phragmites australis</Emphasis> periphyton communities in two Hungarian soda ponds |
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Authors: | Anna Rusznyák Péter Vladár Gitta Szabó Károly Márialigeti Andrea K Borsodi |
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Institution: | 1.Department of Microbiology,E?tv?s Loránd University,Budapest,Hungary |
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Abstract: | Bacterial diversity of reed (Phragmites australis) periphyton communities of Kelemen-szék and Nagy-Vadas (two Hungarian soda ponds) was investigated using molecular cloning
and cultivation-based techniques. The majority of the 80 Kelemen-szék and 72 Nagy-Vadas bacterial isolates proved to be moderately
halophilic and alkaliphilic. A great proportion of the isolates showed phosphatase and urease activity, utilized aesculin,
citrate and certain biopolymers (e.g., gelatine and tween 80). Partial 16S rDNA sequence analysis of 33 Kelemen-szék and 20
Nagy-Vadas ARDRA group representatives showed Gram-positive (Nesterenkonia, Cellulomonas, Dietzia, Bacillus and Planococcus) dominance at both sampling sites. Species of the genera Acidovorax, Hydrogenophaga (β-Proteobacteria) and Flavobacterium, Sphingobacterium (Bacteroidetes) were represented only from Kelemen-szék. Altogether 16 isolates showed low sequence similarity with yet described
bacteria and may represent novel taxa. Screening of the 16S rRNA gene libraries of 129 Kelemen-szék and 158 Nagy-Vadas clones
resulted in 30 and 28 different ARDRA groups, respectively. Sequence analysis revealed a Gram-negative (Rheinheimera, Aquimonas, Cellvibrio, Flavobacterium and Sphingobacterium) dominated phylogenetic diversity. A high number of the clones were affiliated with uncultured bacterial clones described
from diverse environmental samples. |
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Keywords: | Phragmites australis Biofilm Cultivation Cloning 16S rRNA sequence analysis |
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