An alginate-layer technique for culture of Brassica oleracea L. protoplasts |
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Authors: | Agnieszka Kie?kowska Adela Adamus |
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Institution: | (1) Department of Genetics, Plant Breeding and Seed Science, University of Agriculture in Krakow, Al. 29-Listopada 54, 31-425 Krakow, Poland |
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Abstract: | Ten accessions belonging to the Brassica oleracea subspecies alba and rubra, and to B. oleracea var. sabauda were used in this study. Protoplasts were isolated from leaves and hypocotyls of in vitro grown plants. The influence of selected factors on the yield, viability, and mitotic activity of protoplasts immobilized
in calcium alginate layers was investigated. The efficiency of protoplast isolation from hypocotyls was lower (0.7 ± 0.1 × 106 ml−1) than for protoplasts isolated from leaf mesophyll tissue (2 ± 0.1 × 106 ml−1). High (70–90%) viabilities of immobilized protoplasts were recorded, independent of the explant sources. The highest proportion
of protoplasts undergoing divisions was noted for cv. Reball F1, both from mesophyll (29.8 ± 2.2%) and hypocotyl (17.5 ± 0.3%)
tissues. Developed colonies of callus tissue were subjected to regeneration and as a result plants from six accessions were
obtained. |
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