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| 影响小麦成熟胚培养及植株再生因素的研究 | |
| 引用本文： | 李新玲,曲敏,闫玉清,徐香玲.影响小麦成熟胚培养及植株再生因素的研究[J].植物研究,2005,25(1):49-52. |
| 作者姓名： | 李新玲 曲敏 闫玉清 徐香玲 |
| 作者单位： | 1. 哈尔滨师范大学生命与环境科学学院, 哈尔滨 150080; 2. 东北林业大学林木遗传育种教研室, 哈尔滨 150040; 3. 哈尔滨商业大学食品工程学院生物系, 哈尔滨 150010 |
| 基金项目： | 省科技厅攻关课题 (GCO4B112 ) |
| 摘 要： | 对3个不同栽培品种小麦的成熟胚进行离体培养,研究影响小麦愈伤组织诱导和植株再生的一些因素。结果表明,东农7742的苗分化率明显高于龙麦9814和龙麦26;高浓度的玉米素可明显提高芽的分化率;附加低浓度NAA的1/2 MS培养基可有效促进生根。可见,基因型对小麦愈伤组织的分化有很大的影响,附加一定的外源激素有利于提高植株的再生频率。 |
| 关 键 词： | 小麦 成熟胚 愈伤组织 植株再生 |
| 收稿时间： | 2004-11-02 |
| 修稿时间： | 2004年11月2日 |
Study on the factors of wheat mature embryos culture and plant regeneration |
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| LI Xin Ling , QU Min YAN Yu Qing XU Xiang Ling.Study on the factors of wheat mature embryos culture and plant regeneration[J].Bulletin of Botanical Research,2005,25(1):49-52. | |
| Authors: | LI Xin Ling QU Min YAN Yu Qing XU Xiang Ling |
| Institution: | 1. School of Life and Environmental Science, Harbin Normal University, Harbin 150080; 2. Teaching and Research Section of Forestry Genetics and Breeding, Northeast Forestry University, Harbin 150040; 3. Biology department of Food Engineering College, Harbin University of Commerce, Harbin 150010 |
| Abstract: | To culture wheat mature embryos from three cultivars in vitro and study the factors of wheat callus induction and plant regeneration. The result of research shows bud differentiation rate of dongnong 7742 is much higher than longmai 9814 and longmai 26. ZT with high concentration could increase the rate of bud differentiation. The bud formation could be stimulated by half amount of culture medium together with NAA of low concentration. So the genotypes have great influence on wheat callus differentiation and to add certain amount of hormone could increase the rate of plant regeneration. |
| Keywords: | wheat mature embryos callus plant regeneration |
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