| 小鼠腺苷脱氨酶mRNA特异Ribozyme计算机设计 |
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| 引用本文： | 陈桦,陈农安,陆长德,祁国荣.小鼠腺苷脱氨酶mRNA特异Ribozyme计算机设计[J].生物化学与生物物理进展,1994,21(5):460-463. |
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| 作者姓名： | 陈桦 陈农安 陆长德 祁国荣 |
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| 作者单位： | 中国科学院上海生物化学研究所;中国科学院上海生物化学研究所;中国科学院上海生物化学研究所;中国科学院上海生物化学研究所 |
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| 基金项目： | “863”和国家博士后科学基金资助项目. |
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| 摘 要： | 小鼠腺苷脱氨酶mRNA经计算机分析,包括:ribozyme切点位置选择,二级结构预测,基因生物学功能和基因同源性分析,筛选出四个锤头结构ribozyme.结果表明上述ribozyme底物切点两翼碱基形成发夹结构,切点位于单链环区,切点所在基因片段位于该基因生物功能区内,并同已知小鼠其它基因不同源.
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| 关 键 词： | 腺苷脱氨酶mRNA ribozyme 计算机设计 |
| 收稿时间： | 1993/10/26 0:00:00 |
| 修稿时间： | 3/4/1994 12:00:00 AM |
Computer Design of Murine Adenosine Deaminase mRNA Specific Ribozyme |
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| Chen Hu,Chen Nongan,Lu Changde and Qi Guorong.Computer Design of Murine Adenosine Deaminase mRNA Specific Ribozyme[J].Progress In Biochemistry and Biophysics,1994,21(5):460-463. |
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| Authors: | Chen Hu Chen Nongan Lu Changde and Qi Guorong |
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| Institution: | Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Biochemistry, Academia Sinica |
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| Abstract: | With computer analysis of the adenosinedeaminase (ADA) mRNA, which includedcleavage site selection, secondary structureanalysis, biological function and homologyanalysis of the gene fragment around thecleavage site. four hammerhead ribozymeswere designed. These ribozymes targeted se-quence around the cleavage site have hairpinestructure in which the cleavage site is in thering part. This gene fragment is of biologicalimportance in ADA gene. No homologies werefaund between these gene fragments and othermurine genes. These characteristics of thegene fragment make them easy to be pairedand cleaved by their respective ribozymes. |
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| Keywords: | adenosine deaminase ribozyme computer design |
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