Recent advances in DNA sequencing by End-Labeled Free-Solution Electrophoresis (ELFSE) |
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Authors: | Jong-In Won |
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Institution: | (1) Chemistry Department, University of Washington, Seattle, WA 98195-1700, USA;(2) Chemistry Department, University of Alberta, Edmonton, AB, T6G 2M7, Canada;(3) Present address: Midwest Energy Group, INC, Carbondale, IL 62903, USA;(4) Present address: Great Southern Laboratories, Houston, TX 77099, USA; |
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Abstract: | End-Labeled Free-Solution Electrophoresis (ELFSE) is a new technique that is a promising bioconjugate method for DNA sequencing
(or separation) and genotyping by both capillary and microfluidic device electrophoresis. Because ELFSE enables high-resolution
electrophoretic separation in aqueous buffer alone (i.e., without a polymer matrix), it eliminates the need to load viscous polymer networks into electrophoresis microchannels. To
achieve microchannel DNA separations with high performance, ELFSE requires monodisperse perturbing entities (i.e., drag-tags), which create a large amounts of frictional drag when pulled behind DNA during free-solution electrophoresis,
and which have other properties suitable for microchannel electrophoresis. In this article, the theoretical concepts of ELFSE
and the required characteristics of the drag-tag molecules for the ultimate performance of ELFSE are reviewed. Additionally,
the merits and limitations of current drag-tags are also discussed in the context of recent experimental data of ELFSE separation
(or sequencing). |
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