An 83-kDa embryonic-type nuclear antigen is detected within the germinal vesicles of oocytes of the ascidianHalocynthia roretzi |
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| Authors: | Shigeki Fujiwara Noriyuki Satoh |
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| Institution: | (1) Department of Zoology, Faculty of Science, Kyoto University, Sakyo-ku, 606 Kyoto, Japan;(2) Asamushi Marine Biological Station, Tohoku University, 039-34 Aomori, Japan;(3) Present address: Department of Biology, Faculty of Science, Kochi University, 780 Kochi, Japan |
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| Abstract: | Summary Monoclonal antibodies were raised against germinal vesicles which were isolated from fully grown oocytes of the ascidianHalocynthia roretzi. Immunoblot analyses revealed that one of the antibodies, designated Hgv-2, recognized a single band with a molecular weight of about 83 kDa. The antibody, visualized by indirect immunohistochemistry, reacted only with the germinal vesicles of oocytes and did not react with test cells, follicle cells, and other somatic cells of the gonad. During embryogenesis the antigenicity was found in interphase nuclei of all embryonic cells. The antibody did not react with chromosomes or the mitotic apparatus. The antigenicity was retained by interphase nuclei of larval cells, but it disappeared from nuclei of juveniles about 7 days after metamorphosis. |
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| Keywords: | 83-kDa nuclear antigen Germinal vesicles Ascidian embryogenesis Monoclonal antibody |
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