Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding |
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Authors: | Wei Li Cailin Lei Zhijun Cheng Yulin Jia Dongyi Huang Jiulin Wang Jiankang Wang Xin Zhang Ning Su Xiuping Guo Huqu Zhai Jianmin Wan |
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Institution: | 1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China 2. Institute of Crop Science, Chinese Academy of Agricultural Sciences and the National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, 100081, China 3. Agronomy College, Guangdong Ocean University, Zhanjiang, 524088, China 4. USDA-ARS Dale Bumpers National Rice Research Center, 2890 HWY 130 E, Stuttgart, AR, 72160, USA 5. Agronomy College, Hainan University, Danzhou, 571737, China
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Abstract: | The Pi20(t) gene was determined to confer a broad-spectrum resistance against diverse blast pathotypes (races) in China based on inoculation
experiments utilizing 160 Chinese Magnaporthe oryzae (formerly Magnaporthe grisea) isolates, among which isolate 98095 can specifically differentiate the Pi20(t) gene present in cv. IR24. Two flanking and three co-segregating simple sequence repeat (SSR) markers for Pi20(t), located near the centromere region of chromosome 12, were identified using 526 extremely susceptible F2 plants derived from a cross of Asominori, an extremely susceptible cultivar, with resistant cultivar IR24. The SSR OSR32
was mapped at a distance of 0.2 cM from Pi20(t), and the SSR RM28050 was mapped to the other side of Pi20(t) at a distance of 0.4 cM. The other three SSR markers, RM1337, RM5364 and RM7102, co-segregated with Pi20(t). RM1337 and RM5364 were found to be reliable markers of resistance conditioned by Pi20(t) in a wide range of elite rice germplasm in China. As such, they are useful tags in marker-assisted rice breeding programs
aimed at incorporating Pi20(t) into advanced rice breeding lines and, ultimately, at obtaining a durable and broad spectrum of resistance to M. oryaze.
Wei Li and Cailin Lei contributed equally to this work. |
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Keywords: | Gene mapping Magnaporthe oryzae Marker-assisted selection Oryza sativa L Resistance gene Rice blast |
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