Citrate transporters play an important role in regulating aluminum-induced citrate secretion in Glycine max |
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Authors: | J F You N N Hou M Y Xu H M Zhang and Z M Yang |
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Institution: | (1) College of Agriculture, Guangxi University, Nanning, 530005, China;(2) College of Animal Science and Technology, Southwest University, Beibei Chongqing, 400716, China |
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Abstract: | To further understand the process of Al-induced citrate secretion from soybean roots, the effect of protein synthesis inhibitor,
anion channel blockers, and citrate carrier inhibitors on Al-induced citrate exudation was investigated in Al-resistant soybean
cultivar PI 416937. Citrate exudation from roots increased with the increase of Al concentration from 10 to 50 μM and initiated
after 4 h of Al exposure. Protein synthesis inhibitor, cycloheximide (CHM; 25 μM) completely inhibited Al-induced citrate
secretion during 12-h exposure, suggesting that novel protein synthesis was necessary in Al-induced citrate efflux. Also both
anion channel blocker anthracene-9-carboxylic acid (A-9-C) and citrate carrier inhibitor mersalyl acid (Mersalyl) significantly
reduced citrate secretion, suggesting that both anion channels in plasma membrane and citrate carriers in mitochondria membrane
were the rate limiting factors of Al dependent citrate release. However, Al-induced citrate secretion was insensitive to anion
channel blockers phenylglyoxal (PG), 4,4′-diisothiocyanostibene-2,2′-disulfonat (DIDS) and citrate carrier inhibitor pyridoxal
5′-P (PP). |
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Keywords: | |
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