转彩色马铃薯StAN1基因的烟草幼苗耐旱性分析

该研究以转彩色马铃薯StAN1基因烟草为材料、野生型烟草(WT)为对照,测定分析转StAN1基因烟草在种子萌发期、幼苗期和苗期对干旱(甘露醇)处理的耐受情况,并对苗期旱热共同胁迫的耐受情况进行测定分析,以探讨彩色马铃薯StAN1基因的功能,为耐旱彩色马铃薯育种提供新路径。结果显示:(1)转StAN1基因烟草鉴定显示,阳性率为82.6%,且转基因烟草的叶片明显变紫,花青素含量极显著高于野生型烟草。(2)在培养基甘露醇浓度为150 mmol/L时,点播在培养基上的转基因烟草种子第5天时的萌发率达到了7%,是野生型烟草萌发率的2.3倍。(3)在甘露醇浓度为0和100 mmol/L的培养基上竖直培养时,转基因烟草的根长分别是野生型烟草的1.46和1.30倍,根长比野生型烟草显著增长。(4)在干旱胁迫下,转基因烟草幼苗叶片中的脯氨酸含量以及超氧化物歧化酶活性均显著高于野生型烟草,丙二醛含量均显著低于野生型烟草。(5)转基因烟草LEA基因和ERF基因在干旱和旱热处理中的相对表达量均高于野生型烟草。研究表明,StAN1基因在提高植物花青素含量的同时也提高了植物的耐旱性。

Analysis about Drought Tolerance of Tobacco Seedlings Transformed with Color Potato StAN1 Gene

In this study, transgenic tobacco with color potato StAN1 gene was used as the material, and the wild type tobacco (WT) was used as the control. The tolerance of the transgenic StAN1 gene tobacco to drought (mannitol) during the seed germination, shoots and seedling stages, and the tolerance to drought and heat common stress in the seedling stage was measured and analyzed, so as to explore the function of the StAN1 gene of colored potato and provide a new path for drought tolerant colored potato breeding. The results showed: (1) the positive rate of StAN1 transgenic tobacco was 82.6%, the leaves of transgenic tobacco were obviously purple, and the anthocyanin content was significantly higher than that of wild type tobacco. (2) When the mannitol concentration in the medium was 150 mmol/L, the germination rate of the transgenic tobacco on the medium reached 7% on the 5th day, which was 2.3 times of wild type tobacco. (3) When cultured vertically on the medium with mannitol concentration of 0 and 100 mmol/L, the root length of transgenic tobacco was 1.46 and 1.30 times of wild type tobacco, which was significantly longer than that of wild type tobacco. (4) Under drought stress, the proline content and superoxide dismutase activity in the leaves of transgenic tobacco seedlings were significantly higher than that of wild type tobacco, and the content of malondialdehyde was significantly lower than that of wild type tobacco. (5) The relative expression levels of LEA and ERF genes in transgenic tobacco were higher than those in wild type tobacco under drought and drought heat treatments. Studies have shown that the StAN1 gene not only increased the anthocyanin content of plants, but also improved the drought tolerance of plants.