Expression of the Lactobacillus casei lactate dehydrogenase gene in Bacillus subtilis |
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| Authors: | Sungmin F. Kim Seung J. Baek M. Y. Pack |
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| Affiliation: | (1) Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, 305-338 Taejon, Korea;(2) Department of Life Sciences, Pohang University of Science and Technology, 790-784 Pohang, Korea |
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| Abstract: | Summary A gene for allosteric lactate dehydrogenase (LDH) of Lactobacillus casei ATCC393 was transferred into Bacillus subtilis. The LDH was produced in a growth-associated type, and comprised up to 40 % of the total cellular protein. The maximum specific activity in the transformant was 208 U/mg protein which was approximately 16 times higher than in L. casei or in the previously constructed Escherichia coli transformant. |
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