| SARS冠状病毒Mc区原核表达及包涵体复性的研究 |
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| 引用本文： | 刘志伟,龙北国,赵卫.SARS冠状病毒Mc区原核表达及包涵体复性的研究[J].微生物学免疫学进展,2007,35(3):12-15. |
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| 作者姓名： | 刘志伟 龙北国 赵卫 |
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| 作者单位： | 1. 南方医科大学附属南方医院检验科,广州,510515
2. 南方医科大学热带医学与公共卫生学院微生物学系,广州,510515 |
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| 摘 要： | 构建SARS冠状病毒M蛋白膜内区(Mc蛋白)基因(Mc区)融合表达载体,表达并复性该蛋白。克隆Mc区,构建融合表达载体pET-32a(+)/Mc,融合表达Mc蛋白。纯化表达蛋白,采用逐步透析、降低蛋白浓度、加入氧化还原剂复性融合蛋白。用复性蛋白免疫兔产生抗血清,复性蛋白与SARS患者血清和健康人血清反应鉴定复性结果。结果显示,复性蛋白免疫家兔后产生抗血清,该复性蛋白与SARS患者血清特异结合,表明已成功复性该表达蛋白。为SARS疫苗和诊断试剂的研制奠定基础。
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| 关 键 词： | SARS冠状病毒 Mc蛋白 Mc区 原核表达 包涵体复性 |
| 文章编号： | 1005-5673(2007)03-0012-04 |
| 修稿时间： | 2007年2月1日 |
The prokaryotic expression of SAPS-CoV Mc region and renaturation of its inclusion bodies |
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| LIU Zhi-wei,LONG Bei-guo,ZHAO Wei.The prokaryotic expression of SAPS-CoV Mc region and renaturation of its inclusion bodies[J].Progress In Microbiology and Immunology,2007,35(3):12-15. |
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| Authors: | LIU Zhi-wei LONG Bei-guo ZHAO Wei |
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| Abstract: | To clone and construct expression vector pET32a(+)/ Mc and then to express and renature the fusion protein.The Mc region was cloned,the fusion expression vector pET32a(+)/ Mc was constructed and the expressed Mc protein was obtained.Mc protein was purified and renatured through gradient dialysis,reducing protein concentration and adding oxido-reduction agent.The antiserumwas prepared through immunizing rabbitswith renatured Mc protein.The results showed that immunonizedrabbits can produce antibodies,the renatured protein can specially react with SARS patients'serum. |
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| Keywords: | SARS-CoV Mc protein Mc region Prokaryotic expression Inclusion renaturation |
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