NADPH-specific and NADH-specific xylose reduction is catalyzed by two separate enzymes in Pachysolen tannophilus |
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Authors: | G. Ditzelmüller E. M. Kubicek-Pranz M. Röhr C. P. Kubicek |
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Affiliation: | (1) Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9, A-1060 Wien, Austria |
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Abstract: | Summary Pachysolen tannophilus contains — in addition to an NADPH-linked xylose reductase — a separate NADH-linked one, in this respect differing from the yeast Pichia stipitis. Both enzyme proteins can conveniently be separated from each other by either ion exchange chromatography or chromatofocusing. |
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