| SCAR标记在黑木耳栽培菌株分类鉴定中的应用 |
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| 引用本文： | 李媛媛,隋玉龙,牛淑力,吴波,宋慧. SCAR标记在黑木耳栽培菌株分类鉴定中的应用[J]. 菌物研究, 2013, 11(3): 182-185,189 |
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| 作者姓名： | 李媛媛 隋玉龙 牛淑力 吴波 宋慧 |
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| 作者单位： | 1. 吉林农业大学生命科学学院,长春,130118
2. 吉林农业大学生命科学学院,长春130118;吉林农业大学食药用菌教育部工程研究中心,长春130118 |
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| 基金项目： | 吉林省世行贷款农产品质量安全项目 |
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| 摘 要： | 应用序列特异性扩增区域（SCAR）标记技术分析50株黑木耳栽培菌株的遗传多样性。在SRAP标记分析过程中发现1条1 200 bp的特异性条带,经回收克隆测序转为SCAR标记。根据序列设计出1对特异性引物,经PCR可以扩增出1 000 bp大小的片段,说明成功构建出了＂黑931＂的指纹图谱。
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| 关 键 词： | 黑木耳 SCAR 指纹图谱 |
Identification of Fifty Starins of Auricularia auricula-judae by SCAR Marker |
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| LI Yuan-yuan,SUI Yu-long,NIU Shu-li,WU Bo,SONG Hui. Identification of Fifty Starins of Auricularia auricula-judae by SCAR Marker[J]. Journal of Fungal Research, 2013, 11(3): 182-185,189 |
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| Authors: | LI Yuan-yuan SUI Yu-long NIU Shu-li WU Bo SONG Hui |
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| Affiliation: | 1.College of Life Science,Jilin Agricultural University,Changchun 130118,China;2.Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118,China) |
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| Abstract: | Polymorphism of fifty strains of Auricularia auricula-judae was analyzed by SCAR（sequence characterized amplified region）.A 1 200 bp loci,founded during the analysis of Auricularia auricula-judae by SRAP,were transformed into SCAR.A special primer pair were designed by primer 5.0 from the specific sequence and a 1 000 bp loci were amplified.The result show that the fingerprint of Hei931was successfully established. |
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| Keywords: | Auricularia auricula-judae SCAR fingerprint |
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