| 携带三种报告基因慢病毒载体的构建及其实验研究 |
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| 引用本文： | 王丽娴,王东阳,李星,夏海滨. 携带三种报告基因慢病毒载体的构建及其实验研究[J]. 中国生物工程杂志, 2011, 31(12): 86-92 |
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| 作者姓名： | 王丽娴 王东阳 李星 夏海滨 |
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| 作者单位： | 陕西师范大学生命科学学院基因治疗研究室 西安 710062 |
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| 基金项目： | 国家自然科学基金资助项目(3087299331070137) |
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| 摘 要： | 慢病毒是逆转录病毒科的一个属.它具有可以感染分裂期及非分裂期细胞、容纳外源性目的基因片段大、目的基因表达时间较长、免疫反应小等诸多优点,因此成为运载目的基因的理想载体而得到广泛的应用.构建了带有3种报告基因即红色荧光蛋白(mCherry)、荧光素酶基因(luciferase)及绿色荧光蛋白(eGFP)的慢病毒载体,其中...
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| 关 键 词： | 慢病毒载体 报告基因 脑胶质瘤 动物模型 |
| 收稿时间： | 2011-08-09 |
| 修稿时间： | 2011-10-04 |
Construction of a Lentivirus Vector Expressing Three Reporter Genes and Its Experimental Study |
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| WANG Li-xian,WANG Dong-yang,LI Xing,XIA Hai-bin. Construction of a Lentivirus Vector Expressing Three Reporter Genes and Its Experimental Study[J]. China Biotechnology, 2011, 31(12): 86-92 |
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| Authors: | WANG Li-xian WANG Dong-yang LI Xing XIA Hai-bin |
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| Affiliation: | WANG Li-xian WANG Dong-yang LI Xing XIA Hai-bin(Laboratory of Gene Therapy,College of Life Sciences,Shaanxi Normal University,Xi'an 710062,China) |
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| Abstract: | Lentiviral is a genus of retroviridae,which has minor immune response and can deliver and express large exogenous gene fragment for longer time.Therefore,it is widely used as an ideal vector for delivering the target gene.One type of lentivirus vector containing three types of reporter gene,e.g.mCherry,luciferase and eGFP was constructed.Mcherry and eGFP can be used for cell line screening in vitro because of its intuitive imaging,while luciferase can be used for detecting the growth and metastasis of the t... |
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| Keywords: | Lentiviral vector Reporter gene Glioblastoma Animal model |
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