| SELDI-TOF-MS技术的改善和优化 |
| |
| 引用本文： | 陈雅斌,兰小鹏.SELDI-TOF-MS技术的改善和优化[J].生命科学研究,2010,14(1):90-94. |
| |
| 作者姓名： | 陈雅斌 兰小鹏 |
| |
| 作者单位： | 1. 福建医科大学,福州总医院,临床医学院,中国福建,福州,350025
2. 福州总医院,全军检验医学研究所,中国福建,福州,350025 |
| |
| 基金项目： | 南京军区医学科学技术研究"十一五"计划 |
| |
| 摘 要： | 表面增强激光解吸电离飞行时间质谱(Surface-enhanced laser desorption/ionization-time of flight,SELDI-TOF-MS)技术是目前蛋白质组学研究较为有效的手段之一,已被广泛的应用于肿瘤、传染性疾病、心血管病和神经系统等疾病的研究.然而SELDI-TOF-MS技术本身存在着一些问题,如分析结果有效性差和实验重复验证率低等,使其不能完全的应用于常规的临床诊断,现有许多研究致力于解决这些问题,如规范化实验室操作、预处理原始标本和数据、提高统计学方法等极大的改善和优化了这项技术.
|
| 关 键 词： | SELDI．TOF—MS技术：改善：优化 |
Improvement and Optimization of SELDI-TOF-MS |
| |
| CHEN Ya-bin,LAN Xiao-peng.Improvement and Optimization of SELDI-TOF-MS[J].Life Science Research,2010,14(1):90-94. |
| |
| Authors: | CHEN Ya-bin LAN Xiao-peng |
| |
| Institution: | CHEN Ya-bin1,LAN Xiao-peng2 (1.Clinical Medicine School,Fuzhou General Hospital,Fujian Medical University,Fuzhou 350025,Fujian,China,2.Institute of Laboratory Medicine,Fuzhou General Hospital of PLA,China) |
| |
| Abstract: | The surface-enhanced laser desorption /ionization-time of flight (SELDI-TOF-MS)is one of the most promising approaches in proteome research.Now it is widely used in some researches,such as cancer,infection disease,angiocardiopathy,nervous system disease,and so on.But some limitations in SELDI-TOF-MS,for example:the effectivity and duplicate validation of the experimental result is low.Further evaluation and optimization are called for before it's application in clinical diagnostics.A lot of researches such ... |
| |
| Keywords: | SELDI-TOF-MS improvement optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
