A potential bacterial biocontrol agent,strain S2V2 against pathogenic marine <Emphasis Type="Italic">Vibrio</Emphasis> in aquaculture |
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Authors: | Alim Isnansetyo Indah Istiqomah Muhtadi Shofihar Sinansari Rudi Kurnia Hernawan Triyanto Jaka Widada |
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Institution: | (1) Laboratory of Fish Diseases, Department of Fisheries, Faculty of Agriculture, Gadjah Mada University, Jl. Flora Bulaksumur, Yogyakarta, 55182, Indonesia;(2) Laboratory of Agricultural Microbiology, Faculty of Agriculture, Gadjah Mada University, Jl. Flora, Bulaksumur, Yogyakarta, 55182, Indonesia |
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Abstract: | We isolated a marine bacterium strain S2V2 which inhibited the growth of pathogenic marine Vibrio spp. The aims of this research were to identify a new antibiotic-producing marine bacterium strain S2V2, and evaluate its
spectrum activity and pathogenic property. Analysis of 16S rDNA sequence placed strain S2V2 in the genus Pseudoalteromonas, but the sequence similarity was low (95.46%) implying the strain might be a new species in this genus. Strain S2V2 inhibited
the growth of 67.9% of 28 Vibrio strains tested. This strain inhibited V. alginolyticus, V. anguillarum, V. fluvialis, V. harveyi, V. metschnikovii, V. splendidus, V. ordalii, V. parahaemolyticus, and V. vulnificus, but inactive against V. campbellii, Aeromonas hydrophyla and Staphylococcus aureus. Strain S2V2 produced extracellular non proteinaceous antibacterial substances. The highest antibacterial activity was found
when strain S2V2 was cultured for 96 h in ZoBell broth medium. An artificial infection to post larvae of Lithopenaeus vanname indicated that strain S2V2 was a non pathogenic bacterium. Non pathogenic property and specific antibacterial activity against
a broad range of fish pathogenic marine Vibrio of strain S2V2 suggest that this strain is a prospective source of unique antibiotic and a potential biocontrol agent in
marine aquaculture. |
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Keywords: | Antibiotic Biocontrol Marine aquaculture Pseudoalteromonas Vibrio |
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