Chronic ethanol administration decreases fatty acyl-CoA desaturase activities in rat liver microsomes |
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Authors: | Shigenobu Umeki Hiroyuki Shiojiri Yoshinori Nozawa |
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Institution: | Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu, Japan |
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Abstract: | The Δ9-desaturase system in liver microsome from rats treated chronically with ethanol was studied. Stearoyl-CoA desaturase activity decreased by 80% and palmitoyl-CoA desaturase activity was not detectable in microsomes from ethanol-fed rats, while activities of electron transport components such as NADH-cytochrome c and NADH-ferricyanide reductases remained unchanged. However, chronic ethanol administration resulted in an adaptive induction of the activity of NADPH-cytochrome c reductase and the contents of cytochrome b5 and P-450. The activity of the terminal component (cyanide-sensitive factor; CSF) of the desaturase system was greatly depressed by ethanol treatment. The NADH/NAD ratio in microsomes of ethanol-fed rats increased over 2-fold. These results suggest that, during chronic ethanol ingestion, decreased activities of Δ9-desaturases are due mainly to a decreased content of the terminal component of the desaturase system. |
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Keywords: | Chronic ethanol administration Fatty acyl-CoA desaturase Cyanide-sensitive factor |
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