丙型肝炎病毒Core与NS3基因的不同融合方式及其表达

丙型肝炎病毒(简称ＨＣＶ)是危害人类健康的传染性疾病,预防该病的传播主要借助于ＨＣＶ血清学诊断来筛选献血员和检测临床标本。从国内外已分离到的ＨＣＶ株得知,ＨＣＶ核心蛋白(Ｃｏｒｅ)和非结构蛋白ＮＳ3含有较强的优势抗原表位,其相应的抗体出现早,阳性率高,已成为目前第二、第三代ＨＣＶ免疫诊断试剂的重要成份[1,2]。通常Ｃｏｒｅ蛋白和ＮＳ3蛋白分别用基因工程方法表达,然后作为固定化抗原,检测人体内ＨＣＶ的抗体。我们对Ｃｏｒｅ和ＮＳ3的两种不同组合方式及其表达的研究,对于发展新的ＨＣＶ诊断试剂很有帮助,现将结果报道如下。1…

Fusion Expression of Hepatitis C Virus Core Region and NS3 Region in E.coli

The gene encoding the core and NS3 proteins of hepatitis C virus was amplified by PCR, respectirely. Two genes were fused and formed the recombinant plamid pHCV CN (Core NS3) and pHCV NC (NS3 Core). The fused plasmid were expressed in E.coli 06. The pHC CN plasmid expressed fussion protein was shown by a major band with a expected molecular weight about 68 kD on SDS PAGE. However, the pHCV NC plasmid expressed fussion protein was 66 kD in molecular weight. The results indicate that the pHCV NC fussion protein differs from the pHCV CN fussion protein in mulecular weight. It suggests that the fusion way is important for the structure of recombinant proteins.