| EB病毒4种反义寡核苷酸片段对鼻咽癌SUNE—1细胞株的影响 |
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| 引用本文： | 朱振宇,周元平.EB病毒4种反义寡核苷酸片段对鼻咽癌SUNE—1细胞株的影响[J].Virologica Sinica,2000,15(3):209-213. |
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| 作者姓名： | 朱振宇 周元平 |
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| 作者单位： | [1]中山医科大学生物化学教研室,广州 [2]中山医科大学附属三院传染科,广州 |
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| 基金项目： | 广东省科委青年基金!(95 0 35 5 )，华南生物中心及广东省卫生厅资助课题 |
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| 摘 要： | 用不同浓度的ＥＢ病毒４种（ＢＨＲＦ１、ＤＮＡ酶、核抗原－１及胸苷激酶）反义寡核苷酸片段，（ａｎｔｉｓｅｎｓｅ ｏｌｉｇｏｄｅｏｘｙｎｕｃｌｅｏｔｉｄｅ，ＡＳＯ）分别与含１０％胎牛血清或不含胎牛血清的人人低分化鼻咽癌细胞株（ＳＵＮＥ－１）共同培养４８ｈ。结果仅适当浓度的ＢＨＲＦ１－ＡＳＯ能引起无血清培养的ＳＵＮＥ－１细胞株增殖明显受抑制，透射电镜及琼脂糖凝胶电泳检测结果发现ＳＵＮＥ－１细胞发生调亡有
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| 关 键 词： | EB病毒 反义寡核苷酸 细胞调亡 |
Four Kinds of EBV-Antisense Oligodeoxynucleotides Effect on Proliferation of EBV Containing SUNE-1 Cell Line |
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| ZHU Zhen yu,ZHOU Yuan ping,ZHANG Qing xiu,GAO Guo quan,DENG Yi qun,MA Juan quan.Four Kinds of EBV-Antisense Oligodeoxynucleotides Effect on Proliferation of EBV Containing SUNE-1 Cell Line[J].中国病毒学(英文版),2000,15(3):209-213. |
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| Authors: | ZHU Zhen yu ZHOU Yuan ping ZHANG Qing xiu GAO Guo quan DENG Yi qun MA Juan quan |
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| Institution: | ZHU Zhen yu,ZHOU Yuan ping*,ZHANG Qing xiu,GAO Guo quan,DENG Yi qun,MA Juan quan |
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| Abstract: | Poorly differentiated nasopharyngeal carcinoma (SUNE 1) cell line was cultured with Epstein Barr virus (EBV) BHRF1, DNase, thymidine kinase (TK) and nuclear antigen 1 (EBNA1) antisense oligodeoxynucleotide (ASO) for 48 h respectively. Inhibited proliferation of SUNE 1 was only observed in the absence of fetal calf serum (FCS) and exposed to EBV BHRF1 ASO. The results from electron microscopy and agarose gel electrophoresis indicated that apoptosis only appeared in SUNE 1 cells exposed to BHRF1 ASO and absence of FCS. The other 3 kinds of EBV ASOs had not the similar effects at the same concentration. |
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| Keywords: | Epstein Barr virus Antisense oligodeoxynucleotide Apoptosis |
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