广州地区急性散发性HEV毒株基因特征和生物学性状的研究

利用ＲＴ－ＰＣＲ技术对我国广州地区急性性戊型肝炎病毒细胞培养分离株Ｇ９３－１、Ｇ９３－２、Ｇ９３－３和Ｇ９３－４基因组聚合酶区部分核苷序列进行检测，ＰＣＲ阳性产物经纯化、克隆后测定。结果Ｇ９３－１、Ｇ９３－３和Ｇ９３－４株病毒的这段序列完全相同，且与我国新疆暴发流行的ＨＥＶ８７Ａ株及ＨＥＶ代表株的同源性为１００％；而Ｇ９３－２株与它们的差异较大，同源性只有７９．９％；但是，它与１９９７年厦门地区急

Study on Genetic and Biological Character of Sporadic Strains of Acute HEV in Guangzhou

Partial nucleotide sequences (location 4522-4761) from ORF1 of sporadic strains (G93 1,G93 2,G93 3 and G93 4) of acute HEV in Guangzhou were detected, the positive PCR products were purified, cloned and sequenced. In the result, the nucleotide sequences of G93 1, G93 3 and G93 4 strain are all the same to each other, and have a homology of 100% with the Xin Jiang 87A and Burma strain respectively. The G93 2 strain is different, the homology of this part of sequence with them is only 79.9%, but it has a homology of 99.2% with X S1 strain obtained from the acute phase serum of a patient with hepatitis E in Xiamen. In addition, the biological character of the G93 2 strain was studied, the result was as same as the 87A strain's. All the results show there is a variant subgenotype of HEV in the South of China besides the similar strain with Xinjiang, but they have the same biological character.