| 中国烟草曲叶病毒广西株的初步研究 |
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| 引用本文： | 张颖,蔡健和.中国烟草曲叶病毒广西株的初步研究[J].Virologica Sinica,2000,15(4):405-408. |
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| 作者姓名： | 张颖 蔡健和 |
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| 作者单位： | [1]中国科学院微生物研究所,北京 [2]广西农业科学院植物保护研究所,南京 |
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| 摘 要： | 双生病毒 (Ｇｅｍｉｎｉｖｉｒｕｓ)是一种具有孪生颗粒形态的单链环状ＤＮＡ植物病毒1] 。根据基因组结构特征及传播介体 ,双生病毒可分为三个亚组1] :亚组Ⅰ双生病毒全部为叶蝉传播的单组份基因组病毒 ,基因组大小在 2 .6～ 2 .8ｋｂ之间 ,其代表病毒是玉米条纹病毒 (ＭＳＶ) ;亚组Ⅱ双生病毒是单组份基因组病毒 ,基因组大小在 2 .7～ 3.0ｋｂ之间 ;亚组Ⅲ双生病毒全部为粉虱 (Ｂｅｍｉｓｉａｔａｂａｃｉ)传播 ,基因组大小为 2 .5～ 2 .8ｋｂ ,除番茄曲叶病毒ＴＬＣＶ ＡＵＳ2 ] 等几个病毒为单组份基因组外 ,大多数亚组Ⅲ双生病病毒…
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| 关 键 词： | 中国烟草 曲叶病毒 广西株 双生病毒 |
Preliminary Study on Chinese Tobacco Leaf Curl Gemnivirus-Guangxi Species |
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| ZHANG Ying ,YIN Qin yan ,LIU Yu le ,CHEN Gang ,CAI Jian he ,TIAN Bo.Preliminary Study on Chinese Tobacco Leaf Curl Gemnivirus-Guangxi Species[J].中国病毒学(英文版),2000,15(4):405-408. |
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| Authors: | ZHANG Ying YIN Qin yan LIU Yu le CHEN Gang CAI Jian he TIAN Bo |
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| Institution: | ZHANG Ying 1,YIN Qin yan 1,LIU Yu le 1,CHEN Gang 1,CAI Jian he 2,TIAN Bo 1 1 |
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| Abstract: | Using DNA of Chinese Tobacco Leaf Curl Virus Guangxi(TbLCV Guangxi)as the template,a 0.4 kb virus specific fragment induding the total common region of the virus and DNA sequence of 52 aa of N terminal of AV1 was obtained by PCR with primers specific to whitefly transmittal gemniviruses,cloned and sequenced.This fragment can basically represent the characteristics of the virus full length genome.The sequence of this fragment is the same as that of chinese tomato yellow leaf curl virus (TYLCV Chi), which indicates that TbLCV Guangxi and TYLCV Chi are the same gemniviruses. According to the sequence of the common region of the 0.4 kb fragment, the virus full length genome DNA was amplified from the infected tobacco plant by PCR with primers back to back. |
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| Keywords: | TbLCV Guangxi TYLV Chi Common region of geminiruses Full length genome of geminiviruses |
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