| 汉滩病毒感染诱导热休克蛋白70表达 |
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| 引用本文： | 叶苓,杨守京.汉滩病毒感染诱导热休克蛋白70表达[J].Virologica Sinica,2000,15(2):106-110. |
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| 作者姓名： | 叶苓 杨守京 |
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| 作者单位： | [1]广州中山医科大学博士后流动站 [2]第四军医大学病理教研室 |
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| 基金项目： | 国家自然科学基金资助项目!(39770 664) |
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| 摘 要： | 为了解汉滩病毒感染后细胞的应激反应及ＨＳＰ７０的表达与病毒复制的关系，在汉滩病毒Ａ９株感染Ｖｅｒｏ－Ｅ６细胞后，用免疫组织化学及核酸分子原位杂交法，对细胞ＨＳＰ７０基因的表达进行了检测。结果表明，汉滩病毒感染细胞４ｈｙ后即可诱导Ｖｅｒｐ－Ｅ６细胞表达ＨＳＰ７０，表达可持续至感染后５ｄ且ＨＳＰ７０在细胞内的分布也有改变。提示汉滩病毒可直接诱导ＨＳＰ７０的高表达。
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| 关 键 词： | 热休克蛋白 汉滩病毒 Vero－E6细胞 应激反应 |
Expression of HSP70 Induced by Infection of Hantaan Virus |
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| YE Ling,YANG Shou jing,LIU Yan fang.Expression of HSP70 Induced by Infection of Hantaan Virus[J].中国病毒学(英文版),2000,15(2):106-110. |
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| Authors: | YE Ling YANG Shou jing LIU Yan fang |
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| Abstract: | In order to investigate the stress response in the cells infected with Hantaan virus and the relationship between the expression of HSP70 and the virus replication, the Vero E6 cells infected with Hantaan virus (A9 strain) were detected by immunohistochemistry and in situ hybridization. The results show that the expression of HSP70 can be induced in the cells 4 hours after Hantaan virus infection and lasted until the fifth days after infection. The distribution of HSP70 also varied. It suggests that the expression of HSP70 can be induced directly by Hantaan virus. |
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| Keywords: | Heat shock proteins Hantaan virus Vero-E6 cells |
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