Inhibition of lymphocyte growth by spermidine in medium containing fetal bovine serum |
| |
Authors: | T Leonard Swanson Gordon E Gibbs |
| |
Institution: | (1) Department of Pediatrics, University of Nebraska, College of Medicine, 68105 Omaha, Nebraska;(2) Department of Surgery, Denver General Hospital, W. 8th Avenue & Cherokee St., 80204 Denver, Colorado |
| |
Abstract: | Summary The effect of spermidine and fetal bovine serum on DNA, RNA, and protein synthesis in phytohemagglutinin-stimulated human
lymphocytes was investigated. At 10−4
M spermidine, DNA, RNA, and protein synthesis ceased and 70% of the original cell population died within 62 hr. Lower spermidine
concentrations had no significant effect on DNA and protein synthesis, but caused an early, unexplained increase in the rate
of RNA synthesis. Heating at 56°C for 30 min had no effect on the plasma amine oxidase activity in fetal bovine and horse
sera but abolished the activity in human plasma. It is concluded that low amounts of aminoaldehydes and acrolein produced
by plasma amine oxidase at spermidine concentrations below 10−4
M do not noticeably alter lymphocyte metabolism. However, the aminoaldehydes and acrolein produced become abruptly cytotoxic
at 10−4
M spermidine.
This work was supported in part by the Cystic Fibrosis Foundation. |
| |
Keywords: | spermidine lymphocytes amine oxidase |
本文献已被 SpringerLink 等数据库收录! |
|