Chromosomal location of isozyme and seed storage protein genes in Dasypyrum villosum (L.) Candargy |
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| Authors: | L Montebove C De Pace C C Jan G T Scarascia Mugnozza C O Qualset |
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| Institution: | (1) Istituto di Biologia Agraria, Università degli Studi della Tuscia, Via S. Camillo de Lellis, I-01100 Viterbo, Italy;(2) USDA-ARS, Department of Agronomy, North Dakota State University, 58105 Fargo, ND, USA;(3) Department of Agronomy and Range Science, University of California, 95616 Davis, CA, USA |
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| Abstract: | Summary The zymogram phenotypes of glucose-phosphate isomerase (GPI), alcohol dehydrogenase-1 (ADH-1), glutamate oxaloacetate transaminase (GOT), superoxide dismutase (SOD), lipoxygenase (LPX), esterase (EST) and the banding patterns of gliadin and glutenin seed storage proteins were determined for Triticum aestivum cv.  Chinese Spring (CS), Dasypyrum villosum, the octoploid amphiploid T. aestivum cv. Chinese Spring D. villosum (CS × v) (2n=8x=56; AABBDDVV), and for five CS-D. villosum disomic addition lines. The genes Gpi-V1, Adh-V1, Got-V2, and Sod-V2 coding for GPI-1, ADH-1, GOT-2, and SOD-2 isozymes were located in D. villosum on chromosome 1V, 4V, 6V, and 7V, respectively. Genes coding for gliadin- and glutenin-like subunits are located in D. villosum chromosomes 1V. There are no direct evidence for chromosomal location of genes coding for GOT-3, EST-1 and LPX-2 isozymes. The linkage between genes coding for glutenin-like proteins and GPI-1 isozymes in chromosome 1V is evidence of homoeology between chromosome 1V and the chromosomes of homoeologous group 1 in wheat.Research supported by the National Research Council (Italy) and National Science Foundation (USA). International cooperative project, Grant No. 85.01504.06 (CNR) |
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| Keywords: | Isozymes Gliadins Glutenins Gene markers Dasypyrum |
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