| 微流控振荡流PCR快速检测单增李斯特氏菌 |
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| 引用本文： | 章春笋,王海英,李彧媛.微流控振荡流PCR快速检测单增李斯特氏菌[J].激光生物学报,2011,20(3):398-403. |
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| 作者姓名： | 章春笋 王海英 李彧媛 |
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| 作者单位： | 华南师范大学激光生命科学教育部重点实验室暨激光生命科学研究所,广东广州,510631 |
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| 基金项目： | 国家自然科学基金项目，国家自然科学基金-广东联合基金重点项目，科技部国家重点基础研究973计划项目，教育部"长江学者与创新团队计划"创新团队项目 |
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| 摘 要： | 一种高通量振荡流PCR微流控技术已被成功开发,借此来快速检测食品致病菌-单增李斯特氏菌(L.monocytogenes).该PCR微流控装置主要由基于LabView的温度控制与采集系统、三个铜加热块以及聚四氟乙烯(PTFE)毛细管等构成.在该微流控装置上,三个铜块维持PCR反应所需要的三个温度,而包埋在铜块沟槽中的PT...
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| 关 键 词： | 连续流动PCR 振荡流PCR 食品致病菌 单增李斯特氏菌 |
Detection of Listeria monocytogenes Using an Oscillatory-flow PCR Microfluidics |
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| ZHANG Chun-sun,WANG Hai-ying,LI Yu-yuan.Detection of Listeria monocytogenes Using an Oscillatory-flow PCR Microfluidics[J].ACTA Laser Biology Sinica,2011,20(3):398-403. |
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| Authors: | ZHANG Chun-sun WANG Hai-ying LI Yu-yuan |
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| Institution: | ZHANG Chun-sun,WANG Hai-ying,LI Yu-yuan (MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science,South China Normal University,Guangzhou 510631,Guangdong,China) |
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| Abstract: | An oscillatory-flow PCR microfluidics has been sucessfully developed for rapid detection of foodborne bacterial pathogens(Listeria monocytogene).The device mainly consists of the LabView-based temperature control and measurement system,three pieces of copper block and polytetrafluoroethylene(PTFE) capillary.On this device,three copper blocks provided the temperatures for PCR,and the PTFE capillary was embedded in three copper blocks as the PCR reaction channel.DNA amplifications were performed within indivi... |
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| Keywords: | continuous-flow PCR oscillatory-flow PCR foodborne pathogens Listeria monocytogene |
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