Dissolved oxygen as principal parameter for conidia production of biocontrol fungi Trichoderma viride in non-Newtonian wastewater |
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Authors: | M Verma Satinder K Brar R D Tyagi R Y Surampalli J R Valéro |
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Institution: | (1) INRS-ETE, Université du Québec, 490, de la Couronne, Quebéc, Canada, G1K 9A9;(2) US EPA, P.O. Box-17-2141, Kansas City, KS 66117, USA;(3) NRCan Canadian Forestry Services, Laurentian Forestery Centre des 1055 du P.E.P.S, P.O. Box 3800, Québec, Canada, G1V 4C7 |
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Abstract: | Dissolved oxygen (DO) concentration was selected as a principal parameter for translating results of shake flask fermentation of Trichoderma viride (biocontrol fungi) to a fermenter scale. All fermentations were carried out in a 7.5 l automated fermenter with a working volume of 4 l. Fermentation performance parameters such as volumetric oxygen transfer coefficient (k
L
a), oxygen uptake rate (OUR), rheology, conidia concentration, glucose consumption, soluble chemical oxygen demand, entomotoxicity and inhibition index were measured. The conidia concentration, entomotoxicity and inhibition index were either stable or improved at lower DO concentration (30%). Variation of OUR aided in assessing the oxygen supply capacity of the fermenter and biomass growth. Meanwhile, rheological profiles demonstrated the variability of wastewater during fermentation due to mycelial growth and conidiation. In order to estimate power consumption, the agitation and the aeration requirements were quantified in terms of area under the curves, agitation vs. time (rpm h), and aeration vs. time (lpm h). This simple and novel strategy of fermenter operation proved to be highly successful which can be adopted to other biocontrol fungi. |
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Keywords: | Biocontrol agents Conidia Dissolved oxygen Rheology Starch industry wastewater Trichoderma viride |
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