Illegitimate recombination mediated by T4 DNA topoisomerase in vitro |
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Authors: | Hideo Ikeda |
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Institution: | (1) Department of Molecular Biology, The Institute of Medical Science, The University of Tokyo, P.O. Takanawa, 108 Tokyo, Japan |
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Abstract: | Summary Illegitimate recombination dependent on T4 DNA topoisomerase in a cell-free system has recently been described. In that work, recombinants between two phage DNA molecules were produced by the topoisomerase alone, without an Escherichia coli extract. In this paper, it is shown that recombination between phage and circular plasmid DNA molecules can also be detected in the presence or absence of an E. coli extract but at frequencies two or three orders of magnitude lower than that observed in the phage-phage cross. The frequency is probably lower because multiple recombination is required in the case of the phage-plasmid cross. |
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Keywords: | Bacteriophage " target="_blank">gif" alt="lambda" align="BASELINE" BORDER="0"> Deletion Insertion Multiple recombination Subunit-exchange model |
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