Purification of urokinase by monoclonal antibody affinity chromatography |
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| Authors: | P. Hérion A. Bollen |
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| Affiliation: | 1. Department of Molecular Biology, University of Brussels, B-1640, Rhode-St-Genèse, Belgium
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| Abstract: | Matrix-bound monoclonal antibodies against urokinase have been used to purify this enzyme by affinity chromatography. In a single-step procedure, urokinase can be isolated from crude preparations with high yield and high purity, and without loss of enzymatic activity. |
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