Utilizing high-throughput experimentation to enhance specific productivity of an <Emphasis Type="Italic">E.coli</Emphasis>T7 expression system by phosphate limitation |
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Authors: | Robert Huber Simon Roth Natalie Rahmen Jochen Büchs |
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Institution: | (1) AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D-52074 Aachen, Germany |
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Abstract: | Background The specific productivity of cultivation processes can be optimized, amongst others, by using genetic engineering of strains,
choice of suitable host/vector systems or process optimization (e.g. choosing the right induction time). A further possibility
is to reduce biomass buildup in favor of an enhanced product formation, e.g. by limiting secondary substrates in the medium,
such as phosphate. However, with conventional techniques (e.g. small scale cultivations in shake flasks), it is very tedious
to establish optimal conditions for cell growth and protein expression, as the start of protein expression (induction time)
and the degree of phosphate limitation have to be determined in numerous concerted, manually conducted experiments. |
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Keywords: | |
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