| Novel polymorphic microsatellite markers developed in the cotton bollworm Helicoverpa armigera （Lepidoptera：Noctuidae） |
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| 引用本文： | YA-JIE JI YU-CHUN WU DE-XING ZHANG. Novel polymorphic microsatellite markers developed in the cotton bollworm Helicoverpa armigera （Lepidoptera：Noctuidae）[J]. Insect Science, 2005, 12(5): 331-334. DOI: 10.1111/j.1005-295X.2005.00040.x |
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| 作者姓名： | YA-JIE JI YU-CHUN WU DE-XING ZHANG |
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| 作者单位： | [1]State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences [2]Graduate School of the Chinese Academy of Sciences, Beijing, China |
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| 基金项目： | Acknowledgments This work was supported by NSFC and M0ST grants （30025008, G2000016207） and the CAS “Bai Ren Ji Hua” professorship to DXZ. Individual contribution： YJJ, construction and screening of genomic library, primers design and preparation of the manuscript; WYC, testing and optimization of primers, genotyping and data analysis; DXZ, supervision and manuscript preparation. |
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| 摘 要： |
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| 关 键 词： | 基因多态性 棉螟蛉 等位基因 染色体 |
| 收稿时间： | 2005-07-11 |
| 修稿时间： | 2005-07-11 |
Novel polymorphic microsatellite markers developed in the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) |
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| YA-JIE JI,YU-CHUN WU,DE-XING ZHANG. Novel polymorphic microsatellite markers developed in the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae)[J]. , 2005, 12(5): 331-334. DOI: 10.1111/j.1005-295X.2005.00040.x |
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| Authors: | YA-JIE JI YU-CHUN WU DE-XING ZHANG |
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| Affiliation: | State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China;and Graduate School of the Chinese Academy of Sciences, Beijing, China |
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| Abstract: | A novel set of five polymorphic di- or trinucleofide microsatellite loci suitable for population genetic study were developed from an enriched genomic library for the pest insect cotton bollworm, Helicoverpa armigera, and cross-amplifiability of these and other published loci was tested in a closely related species, the tobacco budworm, H.assulta. The expected heterozygosity at these loci ranges from 0.62 to 0.91 in the cotton bollworm. The observed allele numbers varies from 4 to 12 in the limited number of individuals tested. Although a large proportion of cloned microsatellite sequences are present in multi-copy in the cotton bollworm genome, the overwhelming majority of the finalized polymorphic diallelic loci are tri-nucleotide microsatellites - an unexpected outcome, which should facilitate subsequent genotyping analysis. |
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| Keywords: | Helicoverpa armigera microsatellite moth PCR primer multi-copy loci population dynamics |
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