Transgenic plants of ramie (Boehmeria nivea Gaud.) obtained by Agrobacterium mediated transformation |
| |
| Authors: | Diva Maria A Dusi Manuel Dubald Elionor Rita P de Almeida Linda S Caldas Eugen S Gander |
| |
| Institution: | (1) EMBRAPA/CENARGEN, SAIN Parque Rural, C.P. 02372, 70849-970 Brasília DF, Brasil;(2) Departamento de Botânica, Universidade de Brasilia, 70919 Brasília-DF, Brasil |
| |
| Abstract: | A regeneration and transformation protocol for ramie (Boehmeria nivea Gaud.) is presented. Regeneration was obtained from leaf discs placed on solid B-5 medium (Gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. Co-cultivation of leaf discs with Agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by Southern blot and analysis of expression of the GUS-marker gene. |
| |
| Keywords: | Ramie Regeneration Transformation |
| 本文献已被 SpringerLink 等数据库收录！ |
