Population growth and allergen accumulation of <Emphasis Type="Italic">Dermatophagoides pteronyssinus</Emphasis> cultured at 20 and 25°C |
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Authors: | Lakshmi Yella Marjorie S Morgan Larry G Arlian |
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Institution: | (1) Department of Biological Sciences, Wright State University, 3640 Colonel Glenn Highway, Dayton, OH 45435, USA; |
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Abstract: | The house dust mites, Dermatophagoides pteronyssinus and D. farinae are cultured commercially and in research laboratories and material is harvested from these cultures to make extracts that
are used for diagnosis, immunotherapy and research. Temperature and other climatic conditions can influence population growth
rates, dynamics of allergen production, and the associated endotoxin, enzyme and protein levels of the mite material harvested
from these cultures. Here we determined how temperature affected these parameters. Dermatophagoides pteronyssinus was cultured at 20 and 25°C at 75% relative humidity, and at 2-week intervals the concentrations of mites, Der p 1 and Der
p 2 allergens, endotoxin, and selected enzymes were determined. Mite density increased exponentially but growth rate and final
population density were greater at 25°C compared to 20°C. The combined allergen (Der p 1 + Der p 2) concentrations accumulated
in the cultures at about the same rate at both temperatures. However, individual Der p 1 and Der p 2 accumulation rates varied
independently at the two temperatures. Der p 1 accumulated faster at 20°C whereas Der p 2 accumulated faster at 25°C. The
amount of Der p 1 in whole cultures was greater than the amount of Der p 2. The concentration of allergen for washed mites
harvested from the cultures was much less than for the whole cultures. Our study demonstrated that temperature is an important
factor in population growth and the dynamics of allergen production in cultured mites. |
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