Characterization of ferrioxamine E as the principal siderophore ofErwinia herbicola (Enterobacter agglomerans) |
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Authors: | Ingrid Berner Silvia Konetschny-Rapp Günther Jung Günther Winkelmann |
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Institution: | (1) Institut für Biologie 1, Mikrobiologie I, Universität Tübingen, Auf der Morgenstelle, D-7400 Tübingen, Germany;(2) Institut für Organische Chemie, Universität Tübingen, Auf der Morgenstelle, D-7400 Tübingen, Germany |
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Abstract: | Summary Several strains of the enterobacterial groupErwinia herbicola (Enterobacter agglomerans) were screened for siderophore production. After 3 days of growth in a low-iron medium, all strains studied produced hydroxamate siderophores. The retention values of the main siderophore during thin-layer chromatography on silica gel plates and on HPLC reversed-phase columns were identical with those of an authentic sample of ferrioxamine E (norcardamine). Gas-chromatographic analysis of the HI hydrolyzate yielded succinic acid and 1,5-diaminopentane in equimolar amounts; fast-atom-bombardment (FAB) mass spectroscopy showed a molecular mass of 653 Da. Iron from55Fe-labelled ferrioxamine E was well taken up by iron-starved cells ofE. herbicola (K
m=0.1 M,V
max=8 pmol mg–1 min–1). However, besides ferrioxamine E (100%), several exogenous siderophores such as enterobactin (94.5%), ferric citrate (78.5%), coprogen (63.5%) and ferrichrome (17.5%) served as siderophores, suggesting the presence of multiple siderophore receptors in the outer membrane ofE. herbicola. |
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Keywords: | Erwinia herbicola Siderophore transport Ferrioxamine E |
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